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KMID : 0545120090190101201
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 10 p.1201 ~ p.1205
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Effect of Amylose Content in Corn Starch Modification by Thermus aquatiqus 4-α-Glucanotransferase
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Cho Kyoung-Hee
Auh Joong-Hyuck
Ryu Je-Hoon
Park Kwan-Hwa
Park Cheon-Seok
Yoo Sang-Ho
Kim Jung-Hwan
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Abstract
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Corn starches with different amylose contents were enzymatically modified using Thermus aquaticus 4-α-glucanotransferase (TAαGTase). On treating, chain length distribution of the product became broader (degree of polymerization; DP 3-40) after isoamylolysis when compared to the untreated corn starch. In addition, a variety of different size cycloamyloses were formed by glucanotransferring activity of TAαGTase. Cycloamyloses (CAs) with DP 5-40 were detectable in all of the TAαGTase-treated corn starches. The amount of CAs produced by the enzyme treatment seems to increase as amylose content of starch increased when judged by high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC) analyses. Thus, it was suggested that the extent of modification on starch molecules was enhanced in proportion to amylose content of starch by transferring activity of TAαGTase. In turn, it could be useful for developing an efficient process of CA production using this enzyme.
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KEYWORD
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Thermus aquatiqus α-glucanotransferase (TAαGTase), corn starch, amylose, cycloamylose (CA)
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